CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster.

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine miRNA-17-92 (pmiR-17-92) cluster via a CRISPR/Cas9-mediated knock-in strategy. The anti-EGFP or anti-CSFV shRNAs could be stably and effectively expressed at the control of the endogenous promoter of the pmiR-17-92 cluster.

Human Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Hu-96 1 kit of 96 tests
EUR 621.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Human Serum Amyloid P Component (SAP)

Mouse Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Mu-192 1 kit of 192 tests
EUR 798.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Mouse Serum Amyloid P Component (SAP)

Mouse Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Mu-48 1 kit of 48 tests
EUR 358.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Mouse Serum Amyloid P Component (SAP)

Mouse Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Mu-96 1 kit of 96 tests
EUR 469.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Mouse Serum Amyloid P Component (SAP)

Rat Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Ra-192 1 kit of 192 tests
EUR 1183.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Rat Serum Amyloid P Component (SAP)

Rat Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Ra-48 1 kit of 48 tests
EUR 493.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Rat Serum Amyloid P Component (SAP)

Rat Serum Amyloid P Component (SAP) ELISA Kit

DL-SAP-Ra-96 1 kit of 96 tests
EUR 661.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Rat Serum Amyloid P Component (SAP)

Human Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Hu-48T 48T
EUR 498.00
  • Should the Human Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Hu-96T 96T
EUR 647.00
  • Should the Human Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Mu-48T 48T
EUR 381.00
  • Should the Mouse Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Mu-96T 96T
EUR 487.00
  • Should the Mouse Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Ra-48T 48T
EUR 528.00
  • Should the Rat Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit

DLR-SAP-Ra-96T 96T
EUR 690.00
  • Should the Rat Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Human Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Hu-48Tests 48 Tests
EUR 500.00

Human Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Hu-96Tests 96 Tests
EUR 692.00

Mouse Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Mu-48Tests 48 Tests
EUR 368.00

Mouse Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Mu-96Tests 96 Tests
EUR 504.00

Rat Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Ra-48Tests 48 Tests
EUR 534.00

Rat Serum Amyloid P Component (SAP) ELISA Kit

RD-SAP-Ra-96Tests 96 Tests
EUR 742.00

Human Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Hu-48Tests 48 Tests
EUR 522.00

Human Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Hu-96Tests 96 Tests
EUR 724.00

Mouse Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Mu-48Tests 48 Tests
EUR 384.00

Mouse Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Mu-96Tests 96 Tests
EUR 527.00

Rat Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Ra-48Tests 48 Tests
EUR 558.00

Rat Serum Amyloid P Component (SAP) ELISA Kit

RDR-SAP-Ra-96Tests 96 Tests
EUR 776.00

Human Serum Amyloid P Component (SAP) AssayMax ELISA Kit

EA8201-1 96 Well Plate
EUR 396.00

Mouse Serum Amyloid P Component (SAP) AssayMax ELISA Kit

EMA8201-1 96 Well Plate
EUR 417.00

Rat Serum Amyloid P Component (SAP) AssayMax ELISA Kit

ERA8201-1 96 Well Plate
EUR 417.00

SAP-I

H-6940.0001 1.0mg
EUR 126.00
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

SAP-I

H-6940.0005 5.0mg
EUR 418.00
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

SAP 155 Antibody

45386-100ul 100ul
EUR 252.00

SAP 155 Antibody

45386-50ul 50ul
EUR 187.00

SAP 155 Antibody

ABD8604 100 ug
EUR 438.00

anti-SH2D1A/SAP

YF-PA13000 50 ul
EUR 363.00
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP

YF-PA13001 50 ug
EUR 363.00
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP

YF-PA13002 100 ug
EUR 403.00
Description: Rabbit polyclonal to SH2D1A/SAP

SAP 155 Antibody

DF8604 200ul
EUR 304.00
Description: SAP 155 Antibody detects endogenous levels of total SAP 155.

SAP 155 Conjugated Antibody

C45386 100ul
EUR 397.00

Ser-Ala-SAP-IIB

5-01923 4 x 1mg Ask for price

SAP 14 Polyclonal Antibody

ABP52407-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody

ABP52407-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody

ABP52407-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 155 Polyclonal Antibody

ABP52769-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody

ABP52769-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody

ABP52769-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 49 Polyclonal Antibody

ABP53447-003ml 0.03ml
EUR 158.00
  • Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody

ABP53447-01ml 0.1ml
EUR 289.00
  • Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody

ABP53447-02ml 0.2ml
EUR 414.00
  • Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110
  • Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

Mouse SAP ELISA Kit

E-90SAP 1 x 96 well plate
EUR 492.00

SAP 155 Polyclonal Antibody

ES3768-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 155 Polyclonal Antibody

ES3768-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 49 Polyclonal Antibody

ES4446-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

SAP 49 Polyclonal Antibody

ES4446-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

SAP 14 Polyclonal Antibody

ES3406-100ul 100ul
EUR 279.00
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 14 Polyclonal Antibody

ES3406-50ul 50ul
EUR 207.00
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Porcine SAP ELISA Kit

EPS0241 96Tests
EUR 521.00

Sheep SAP ELISA Kit

ESS0241 96Tests
EUR 521.00

Anserini SAP ELISA Kit

EAS0241 96Tests
EUR 521.00

Bovine SAP ELISA Kit

EBS0241 96Tests
EUR 521.00

Anti-SH2D1A/SAP (1C9)

YF-MA10530 100 ug
EUR 363.00
Description: Mouse monoclonal to SH2D1A/SAP

Human SAP ELISA Kit

EHS0241 96Tests
EUR 521.00

Goat SAP ELISA Kit

EGTS0241 96Tests
EUR 521.00

SAP 155 Blocking Peptide

DF8604-BP 1mg
EUR 195.00

Canine SAP ELISA Kit

ECS0241 96Tests
EUR 521.00

Chicken SAP ELISA Kit

ECKS0241 96Tests
EUR 521.00

Mouse SAP ELISA Kit

EMS0241 96Tests
EUR 521.00

Rat SAP ELISA Kit

ERS0241 96Tests
EUR 521.00

Monkey SAP ELISA Kit

EMKS0241 96Tests
EUR 521.00

Rabbit SAP ELISA Kit

ERTS0241 96Tests
EUR 521.00

Polyclonal SAP-97 Antibody

APR11177G 0.05ml
EUR 659.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP-97 . This antibody is tested and proven to work in the following applications:

Polyclonal SAP 102 Antibody

APR13193G 0.05ml
EUR 659.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP 102 . This antibody is tested and proven to work in the following applications:

Saponins vaccine adjuvant Combo Pak-1 (contains 1mg of QS21,100 mg of Vet-SAP and 1 g of Saponin).

AV-4000-PK-1 1 pk
EUR 529.00

Polyclonal SAP Antibody (Center E300)

AMR09812G 0.1ml
EUR 484.00
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP (Center E300). This antibody is tested and proven to work in the following applications:

Guinea Pig SAP ELISA Kit

EGS0241 96Tests
EUR 521.00

Streptavidin-Alkaline Phosphatase conjugate activity Elisa kit, 48 tests, quantitative

80305-SAP 1 Kit
EUR 590.00

Aptide scaffold protein anti-Human HER2

SAP-L048 1 mg
EUR 4496.00
Description: Scaffold protein

Avian pancreatic polypeptide scaffold protein anti-Human CBP

SAP-L244 1 mg
EUR 4496.00
Description: Scaffold protein

Ser-Ala-SAP-IIB (AA: Ser-Ala-Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val

SP-89819-1 1 mg
EUR 164.00

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody

abx028838-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody

abx028838-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody

abx033374-400ul 400 ul
EUR 523.00
  • Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody

abx033374-80l 80 µl
EUR 286.00
  • Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody

20-abx102486
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody

20-abx102487
  • EUR 439.00
  • EUR 133.00
  • EUR 1247.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody

20-abx128577
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody

20-abx129714
  • EUR 314.00
  • EUR 133.00
  • EUR 829.00
  • EUR 439.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

SAP domain-containing ribonucleoprotein (SARNP) Antibody

20-abx328336
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody

20-abx178376
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug
  • Please enquire.

Serum Amyloid P Component (SAP) Antibody

20-abx174527
  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug
  • Please enquire.

Serum Amyloid P Component (SAP) Antibody

20-abx174528
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug
  • Please enquire.

Serum Amyloid P Component (SAP) Antibody

abx237756-100ug 100 ug
EUR 481.00
  • Shipped within 5-12 working days.

Serum Amyloid P Component (SAP) Antibody

abx237757-100ug 100 ug
EUR 481.00
  • Shipped within 5-12 working days.

Mouse SAP/PTX2 PicoKine ELISA Kit

EK1208 96 wells
EUR 425.00
Description: For quantitative detection of mouse SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Rat SAP/PTX2 PicoKine ELISA Kit

EK1209 96 wells
EUR 425.00
Description: For quantitative detection of rat SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Human SAP/PTX2 PicoKine ELISA Kit

EK1557 96 wells
EUR 425.00
Description: For quantitative detection of human SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

ELISA kit for Mouse SAP/PTX2

EK5531 96 tests
EUR 553.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat SAP/PTX2

EK5532 96 tests
EUR 553.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human SAP/PTX2

EK5739 96 tests
EUR 553.00
Description: Enzyme-linked immunosorbent assay kit for quantification of Human SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

Recombinant Serum Amyloid P Component (SAP)

4-RPB539Hu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P02743
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 24.8kDa
  • Isoelectric Point: 6.4
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)

4-RPB539Hu02
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P02743
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 50.0kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous pmiR-17-92 expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. Moreover, these TG PFFs could inhibit the replication of CSFV by half and could be further used for generation of transgenic pigs. Taken together, these results show that our RNA interference (RNAi) expression strategy benefits numerous applications, from miRNA, genome and transgenic research, to gene therapy.