CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster.

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine miRNA-17-92 (pmiR-17-92) cluster via a CRISPR/Cas9-mediated knock-in strategy. The anti-EGFP or anti-CSFV shRNAs could be stably and effectively expressed at the control of the endogenous promoter of the pmiR-17-92 cluster.

Mouse Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Mu-48T	DL Develop	48T	EUR 381
Should the Mouse Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Mu-96T	DL Develop	96T	EUR 487
Should the Mouse Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Ra-48T	DL Develop	48T	EUR 528
Should the Rat Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Ra-96T	DL Develop	96T	EUR 690
Should the Rat Serum Amyloid P Component (SAP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Human Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 522

Human Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 724

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Mu-48Tests	Reddot Biotech	48 Tests	EUR 384

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Mu-96Tests	Reddot Biotech	96 Tests	EUR 527

Rat Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Ra-48Tests	Reddot Biotech	48 Tests	EUR 558

Rat Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Ra-96Tests	Reddot Biotech	96 Tests	EUR 776

Human Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Hu-48Tests	Reddot Biotech	48 Tests	EUR 500

Human Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Hu-96Tests	Reddot Biotech	96 Tests	EUR 692

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Mu-48Tests	Reddot Biotech	48 Tests	EUR 368

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Mu-96Tests	Reddot Biotech	96 Tests	EUR 504

Rat Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Ra-48Tests	Reddot Biotech	48 Tests	EUR 534

Rat Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Ra-96Tests	Reddot Biotech	96 Tests	EUR 742

SAP-I
H-6940.0001	Bachem	1.0mg	EUR 126
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

SAP-I
H-6940.0005	Bachem	5.0mg	EUR 418
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

Human Serum Amyloid P Component (SAP) AssayMax ELISA Kit
EA8201-1	AssayPro	96 Well Plate	EUR 396

Mouse Serum Amyloid P Component (SAP) AssayMax ELISA Kit
EMA8201-1	AssayPro	96 Well Plate	EUR 417

Rat Serum Amyloid P Component (SAP) AssayMax ELISA Kit
ERA8201-1	AssayPro	96 Well Plate	EUR 417

SAP 155 Antibody
45386-100ul	SAB	100ul	EUR 252

SAP 155 Antibody
45386-50ul	SAB	50ul	EUR 187

SAP 155 Antibody
DF8604	Affbiotech	200ul	EUR 304
Description: SAP 155 Antibody detects endogenous levels of total SAP 155.

SAP 155 Antibody
ABD8604	Lifescience Market	100 ug	EUR 438

anti-SH2D1A/SAP
YF-PA13000	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP
YF-PA13001	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP
YF-PA13002	Abfrontier	100 ug	EUR 403
Description: Rabbit polyclonal to SH2D1A/SAP

Ser-Ala-SAP-IIB
5-01923	CHI Scientific	4 x 1mg	Ask for price

Mouse SAP ELISA Kit
E-90SAP	Immunology Consultants Laboratory	1 x 96 well plate	EUR 492

SAP 155 Blocking Peptide
DF8604-BP	Affbiotech	1mg	EUR 195

Human SAP ELISA Kit
EHS0241	Abclonal	96Tests	EUR 521

Bovine SAP ELISA Kit
EBS0241	Abclonal	96Tests	EUR 521

Anserini SAP ELISA Kit
EAS0241	Abclonal	96Tests	EUR 521

Chicken SAP ELISA Kit
ECKS0241	Abclonal	96Tests	EUR 521

Canine SAP ELISA Kit
ECS0241	Abclonal	96Tests	EUR 521

Goat SAP ELISA Kit
EGTS0241	Abclonal	96Tests	EUR 521

Polyclonal SAP-97 Antibody
APR11177G	Leading Biology	0.05ml	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP-97 . This antibody is tested and proven to work in the following applications:

Polyclonal SAP 102 Antibody
APR13193G	Leading Biology	0.05ml	EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP 102 . This antibody is tested and proven to work in the following applications:

SAP 155 Conjugated Antibody
C45386	SAB	100ul	EUR 397

SAP 155 Polyclonal Antibody
ABP52769-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155 Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody
ABP52769-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155 Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody
ABP52769-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from the N-terminal region of human SAP 155 Applications tips:
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 49 Polyclonal Antibody
ABP53447-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110 Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody
ABP53447-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110 Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody
ABP53447-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110 Applications tips:
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 14 Polyclonal Antibody
ABP52407-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140 Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody
ABP52407-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140 Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody
ABP52407-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140 Applications tips:
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody
ES3406-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 14 Polyclonal Antibody
ES3406-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 155 Polyclonal Antibody
ES3768-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 155 Polyclonal Antibody
ES3768-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Porcine SAP ELISA Kit
EPS0241	Abclonal	96Tests	EUR 521

SAP 49 Polyclonal Antibody
ES4446-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

SAP 49 Polyclonal Antibody
ES4446-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

Sheep SAP ELISA Kit
ESS0241	Abclonal	96Tests	EUR 521

Rat SAP ELISA Kit
ERS0241	Abclonal	96Tests	EUR 521

Rabbit SAP ELISA Kit
ERTS0241	Abclonal	96Tests	EUR 521

Monkey SAP ELISA Kit
EMKS0241	Abclonal	96Tests	EUR 521

Mouse SAP ELISA Kit
EMS0241	Abclonal	96Tests	EUR 521

Anti-SAP 14 antibody
STJ95575	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to SAP 14.

Anti-SAP 49 antibody
STJ95576	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to SAP 49.

Anti-SAP 155 antibody
STJ96403	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to SAP 155.

Anti-SH2D1A / SAP antibody
STJ70732	St John's Laboratory	100 µg	EUR 359

Anti-SH2D1A/SAP (1C9)
YF-MA10530	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to SH2D1A/SAP

Saponins vaccine adjuvant Combo Pak-1 (contains 1mg of QS21,100 mg of Vet-SAP and 1 g of Saponin).
AV-4000-PK-1	Alpha Diagnostics	1 pk	EUR 529

Polyclonal SAP Antibody (Center E300)
AMR09812G	Leading Biology	0.1ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP (Center E300). This antibody is tested and proven to work in the following applications:

Guinea Pig SAP ELISA Kit
EGS0241	Abclonal	96Tests	EUR 521

SAP ELISA Kit (Mouse) (OKCD04464)
OKCD04464	Aviva Systems Biology	96 Wells	EUR 570
Description: Description of target: ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.37 ng/mL

SAP ELISA Kit (Mouse) (OKIA00113)
OKIA00113	Aviva Systems Biology	96 Wells	EUR 727
Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.845 ng/ml

Streptavidin-Alkaline Phosphatase conjugate activity Elisa kit, 48 tests, quantitative
80305-SAP	Alpha Diagnostics	1 Kit	EUR 590

Aptide scaffold protein anti-Human HER2
SAP-L048	Creative Biolabs	1 mg	EUR 4496
Description: Scaffold protein

Avian pancreatic polypeptide scaffold protein anti-Human CBP
SAP-L244	Creative Biolabs	1 mg	EUR 4496
Description: Scaffold protein

Ser-Ala-SAP-IIB (AA: Ser-Ala-Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val
SP-89819-1	Alpha Diagnostics	1 mg	EUR 164

Polyclonal Goat Anti-SH2D1A / SAP Antibody
APG00306G	Leading Biology	0.1mg	EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-SH2D1A / SAP . This antibody is tested and proven to work in the following applications:

Serum Amyloid P Component (SAP) Antibody
20-abx178376	Abbexa	EUR 1177.00 EUR 578.00	1 mg 200 ug
Please enquire.

Serum Amyloid P Component (SAP) Antibody
20-abx102486	Abbexa	EUR 411.00 EUR 133.00 EUR 1149.00 EUR 565.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody
20-abx102487	Abbexa	EUR 439.00 EUR 133.00 EUR 1247.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody
20-abx128577	Abbexa	EUR 411.00 EUR 133.00 EUR 1149.00 EUR 565.00 EUR 314.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody
20-abx129714	Abbexa	EUR 314.00 EUR 133.00 EUR 829.00 EUR 439.00 EUR 272.00	100 ug 10 ug 1 mg 200 ug 50 ug
Shipped within 5-7 working days.

Serum Amyloid P Component (SAP) Antibody
20-abx174527	Abbexa	EUR 815.00 EUR 425.00	1 mg 200 ug
Please enquire.

Serum Amyloid P Component (SAP) Antibody
20-abx174528	Abbexa	EUR 871.00 EUR 453.00	1 mg 200 ug
Please enquire.

Serum Amyloid P Component (SAP) Antibody
abx033374-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody
abx033374-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody
abx028838-400ul	Abbexa	400 ul	EUR 523
Shipped within 5-10 working days.

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody
abx028838-80l	Abbexa	80 µl	EUR 286
Shipped within 5-10 working days.

Serum Amyloid P Component (SAP) Antibody
abx237756-100ug	Abbexa	100 ug	EUR 481
Shipped within 5-12 working days.

Serum Amyloid P Component (SAP) Antibody
abx237757-100ug	Abbexa	100 ug	EUR 481
Shipped within 5-12 working days.

ELISA kit for Mouse SAP/PTX2
EK5531	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat SAP/PTX2
EK5532	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human SAP/PTX2
EK5739	SAB	96 tests	EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse SAP/PTX2 PicoKine ELISA Kit
EK1208	BosterBio	96 wells	EUR 425
Description: For quantitative detection of mouse SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Rat SAP/PTX2 PicoKine ELISA Kit
EK1209	BosterBio	96 wells	EUR 425
Description: For quantitative detection of rat SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Human SAP/PTX2 PicoKine ELISA Kit
EK1557	BosterBio	96 wells	EUR 425
Description: For quantitative detection of human SAP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

SAP domain-containing ribonucleoprotein (SARNP) Antibody
20-abx328336	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug
Shipped within 5-10 working days.

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Hu01	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P02743 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 24.8kDa Isoelectric Point: 6.4
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Hu02	Cloud-Clone	EUR 476.32 EUR 230.00 EUR 1511.20 EUR 570.40 EUR 1040.80 EUR 382.00 EUR 3628.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P02743 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 50.0kDa Isoelectric Point: Inquire
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Mu01	Cloud-Clone	EUR 413.60 EUR 214.00 EUR 1276.00 EUR 492.00 EUR 884.00 EUR 340.00 EUR 3040.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P12246 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 25.5kDa Isoelectric Point: Inquire
Description: Recombinant Mouse Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Ra01	Cloud-Clone	EUR 503.20 EUR 238.00 EUR 1612.00 EUR 604.00 EUR 1108.00 EUR 400.00 EUR 3880.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Uniprot ID: P23680 Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. Form: Freeze-dried powder Predicted Molecular Mass (KD): 24.3kDa Isoelectric Point: 6.4
Description: Recombinant Rat Serum Amyloid P Component expressed in: E.coli

Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous pmiR-17-92 expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. Moreover, these TG PFFs could inhibit the replication of CSFV by half and could be further used for generation of transgenic pigs. Taken together, these results show that our RNA interference (RNAi) expression strategy benefits numerous applications, from miRNA, genome and transgenic research, to gene therapy.