CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster.

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine miRNA-17-92 (pmiR-17-92) cluster via a CRISPR/Cas9-mediated knock-in strategy. The anti-EGFP or anti-CSFV shRNAs could be stably and effectively expressed at the control of the endogenous promoter of the pmiR-17-92 cluster.

Rat Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Ra-48Tests 48 Tests
EUR 534

Rat Serum Amyloid P Component (SAP) ELISA Kit
RD-SAP-Ra-96Tests 96 Tests
EUR 742

Human Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Hu-48T 48T
EUR 498
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Hu-96T 96T
EUR 647
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Mu-48T 48T
EUR 381
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Mu-96T 96T
EUR 487
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid P Component (SAP) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Ra-48T 48T
EUR 528
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Rat Serum Amyloid P Component (SAP) ELISA Kit
DLR-SAP-Ra-96T 96T
EUR 690
Description: A sandwich quantitative ELISA assay kit for detection of Rat Serum Amyloid P Component (SAP) in samples from serum, plasma or other biological fluids.

Human Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Hu-48Tests 48 Tests
EUR 522

Human Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Hu-96Tests 96 Tests
EUR 724

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Mu-48Tests 48 Tests
EUR 384

Mouse Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Mu-96Tests 96 Tests
EUR 527

Rat Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Ra-48Tests 48 Tests
EUR 558

Rat Serum Amyloid P Component (SAP) ELISA Kit
RDR-SAP-Ra-96Tests 96 Tests
EUR 776

SAP-I
H-6940.0001 1.0mg
EUR 126
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

SAP-I
H-6940.0005 5.0mg
EUR 418
Description: Sum Formula: C38H57N11O14; CAS# [76901-59-2]

Human Serum Amyloid P Component (SAP) AssayMax ELISA Kit
EA8201-1 96 Well Plate
EUR 396

Mouse Serum Amyloid P Component (SAP) AssayMax ELISA Kit
EMA8201-1 96 Well Plate
EUR 417

Rat Serum Amyloid P Component (SAP) AssayMax ELISA Kit
ERA8201-1 96 Well Plate
EUR 417

SAP 155 Antibody
45386-100ul 100ul
EUR 252

SAP 155 Antibody
45386-50ul 50ul
EUR 187

SAP 155 Antibody
ABD8604 100 ug
EUR 438

SAP 155 Antibody
DF8604 200ul
EUR 304
Description: SAP 155 Antibody detects endogenous levels of total SAP 155.

anti-SH2D1A/SAP
YF-PA13000 50 ul
EUR 363
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP
YF-PA13001 50 ug
EUR 363
Description: Mouse polyclonal to SH2D1A/SAP

anti-SH2D1A/SAP
YF-PA13002 100 ug
EUR 403
Description: Rabbit polyclonal to SH2D1A/SAP

Sheep SAP ELISA Kit
ESS0241 96Tests
EUR 521

SAP 49 Polyclonal Antibody
ES4446-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

SAP 49 Polyclonal Antibody
ES4446-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against SAP 49 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

SAP 14 Polyclonal Antibody
ES3406-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 155 Polyclonal Antibody
ES3768-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 155 Polyclonal Antibody
ES3768-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against SAP 155 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

SAP 14 Polyclonal Antibody
ES3406-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against SAP 14 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Rat SAP ELISA Kit
ERS0241 96Tests
EUR 521

Rabbit SAP ELISA Kit
ERTS0241 96Tests
EUR 521

Monkey SAP ELISA Kit
EMKS0241 96Tests
EUR 521

Mouse SAP ELISA Kit
EMS0241 96Tests
EUR 521

Porcine SAP ELISA Kit
EPS0241 96Tests
EUR 521

Goat SAP ELISA Kit
EGTS0241 96Tests
EUR 521

Human SAP ELISA Kit
EHS0241 96Tests
EUR 521

Bovine SAP ELISA Kit
EBS0241 96Tests
EUR 521

Chicken SAP ELISA Kit
ECKS0241 96Tests
EUR 521

Canine SAP ELISA Kit
ECS0241 96Tests
EUR 521

Anserini SAP ELISA Kit
EAS0241 96Tests
EUR 521

Polyclonal SAP-97 Antibody
APR11177G 0.05ml
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP-97 . This antibody is tested and proven to work in the following applications:

Polyclonal SAP 102 Antibody
APR13193G 0.05ml
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP 102 . This antibody is tested and proven to work in the following applications:

Ser-Ala-SAP-IIB
5-01923 4 x 1mg Ask for price

SAP 155 Polyclonal Antibody
ABP52769-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody
ABP52769-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 155 Polyclonal Antibody
ABP52769-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of SAP 155 from Human, Mouse. This SAP 155 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human SAP 155

SAP 49 Polyclonal Antibody
ABP53447-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody
ABP53447-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 49 Polyclonal Antibody
ABP53447-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of SAP 49 from Human, Mouse, Rat. This SAP 49 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human SAP 49 at AA rangle: 30-110

SAP 14 Polyclonal Antibody
ABP52407-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody
ABP52407-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

SAP 14 Polyclonal Antibody
ABP52407-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of SAP 14 from Human, Mouse. This SAP 14 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human SAP 14 at AA range: 60-140

Anti-SAP 155 antibody
STJ96403 200 µl
EUR 197
Description: Rabbit polyclonal to SAP 155.

Anti-SAP 14 antibody
STJ95575 200 µl
EUR 197
Description: Rabbit polyclonal to SAP 14.

Anti-SAP 49 antibody
STJ95576 200 µl
EUR 197
Description: Rabbit polyclonal to SAP 49.

Anti-SH2D1A / SAP antibody
STJ70732 100 µg
EUR 359

SAP 155 Conjugated Antibody
C45386 100ul
EUR 397

SAP 155 Blocking Peptide
DF8604-BP 1mg
EUR 195

Anti-SH2D1A/SAP (1C9)
YF-MA10530 100 ug
EUR 363
Description: Mouse monoclonal to SH2D1A/SAP

Mouse SAP ELISA Kit
E-90SAP 1 x 96 well plate
EUR 492

Saponins vaccine adjuvant Combo Pak-1 (contains 1mg of QS21,100 mg of Vet-SAP and 1 g of Saponin).
AV-4000-PK-1 1 pk
EUR 529

Aptide scaffold protein anti-Human HER2
SAP-L048 1 mg
EUR 4496
Description: Scaffold protein

Avian pancreatic polypeptide scaffold protein anti-Human CBP
SAP-L244 1 mg
EUR 4496
Description: Scaffold protein

Streptavidin-Alkaline Phosphatase conjugate activity Elisa kit, 48 tests, quantitative
80305-SAP 1 Kit
EUR 590

Guinea Pig SAP ELISA Kit
EGS0241 96Tests
EUR 521

Polyclonal SAP Antibody (Center E300)
AMR09812G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SAP (Center E300). This antibody is tested and proven to work in the following applications:

SAP ELISA Kit (Mouse) (OKCD04464)
OKCD04464 96 Wells
EUR 570
Description: Description of target: ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.37 ng/mL

SAP ELISA Kit (Mouse) (OKIA00113)
OKIA00113 96 Wells
EUR 727
Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.845 ng/ml

Ser-Ala-SAP-IIB (AA: Ser-Ala-Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val
SP-89819-1 1 mg
EUR 164

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Hu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Hu02
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Mu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Serum Amyloid P Component expressed in: E.coli

Recombinant Serum Amyloid P Component (SAP)
4-RPB539Ra01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Serum Amyloid P Component expressed in: E.coli

Polyclonal Goat Anti-SH2D1A / SAP Antibody
APG00306G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-SH2D1A / SAP . This antibody is tested and proven to work in the following applications:

Serum Amyloid P Component (SAP) Antibody
20-abx128577
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Serum Amyloid P Component (SAP) Antibody
20-abx129714
  • EUR 314.00
  • EUR 133.00
  • EUR 829.00
  • EUR 439.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Serum Amyloid P Component (SAP) Antibody
20-abx102486
  • EUR 411.00
  • EUR 133.00
  • EUR 1149.00
  • EUR 565.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Serum Amyloid P Component (SAP) Antibody
20-abx102487
  • EUR 439.00
  • EUR 133.00
  • EUR 1247.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Serum Amyloid P Component (SAP) Antibody
abx033374-400ul 400 ul
EUR 523

Serum Amyloid P Component (SAP) Antibody
abx033374-80l 80 µl
EUR 286

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody
abx028838-400ul 400 ul
EUR 523

SAP Domain-Containing Ribonucleoprotein (SARNP) Antibody
abx028838-80l 80 µl
EUR 286

Serum Amyloid P Component (SAP) Antibody
abx237756-100ug 100 ug
EUR 481

Serum Amyloid P Component (SAP) Antibody
abx237757-100ug 100 ug
EUR 481

Serum Amyloid P Component (SAP) Antibody
20-abx178376
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Serum Amyloid P Component (SAP) Antibody
20-abx174527
  • EUR 815.00
  • EUR 425.00
  • 1 mg
  • 200 ug

Serum Amyloid P Component (SAP) Antibody
20-abx174528
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug

SAP domain-containing ribonucleoprotein (SARNP) Antibody
20-abx328336
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

SAP/PTX2 ELISA Kit (Rat) (OKBB00541)
OKBB00541 96 Wells
EUR 505
Description: Description of target: Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.;Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 50 pg/mL

ELISA kit for Mouse SAP/PTX2
EK5531 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat SAP/PTX2
EK5532 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human SAP/PTX2
EK5739 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human SAP/PTX2 in samples from serum, plasma, tissue homogenates and other biological fluids.

Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous pmiR-17-92 expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. Moreover, these TG PFFs could inhibit the replication of CSFV by half and could be further used for generation of transgenic pigs. Taken together, these results show that our RNA interference (RNAi) expression strategy benefits numerous applications, from miRNA, genome and transgenic research, to gene therapy.